期刊
NUTRIENTS
卷 13, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/nu13113967
关键词
Shigella dysenteriae; isothiocyanates; sulforaphane; phenethyl isothiocyanate; stringent response; Galleria mellonella
资金
- National Science Center, Poland [2013/11/B/NZ2/02582, 2016/21/B/NZ7/02077]
- Foundation for Polish Science, Poland [START 059.2017 *7E9]
- University of Gdansk
Cruciferous vegetables are a rich source of organosulfur compounds that have proven health benefits. Isothiocyanate derivatives, such as SFN and PEITC, have shown effective antibacterial potency against Shigella dysenteriae, negatively regulating the expression of the stx gene. This suggests that isothiocyanates can be effectively used to combat dangerous bacterial infections.
Cruciferous vegetables, widely present in daily diets, are a rich source of organosulfur compounds with proven health benefits, especially chemopreventive or antioxidative effects. Isothiocyanate derivatives (ITCs) exhibit a broad spectrum of biological and pharmacological activity and recently, their antibacterial properties have been of particular importance. Here, we have focused on the anti-shigellosis activity of sulforaphane (SFN) and phenethyl ITC (PEITC). The genus Shigella causes gastroenteritis in humans, which constitutes a threat to public health. Production of a potent Stx toxin by S. dysenteriae type 1 results not only in more severe symptoms but also in serious sequela, including the hemolytic uremic syndrome. Here, we present evidence that two aliphatic and aromatic ITCs derivatives, SFN and PEITC, have an effective antibacterial potency against S. dysenteriae, also negatively regulating the stx gene expression. The molecular mechanism of this effect involves induction of the global stress-induced stringent response. ITCs also inhibit bacterial virulence against the Vero and HeLa cells. We present evidence for the therapeutic effect of sulforaphane and phenethyl ITC against a S. dysenteriae infection in the Galleria mellonella larvae model. Thus, our results indicate that isothiocyanates can be effectively used to combat dangerous bacterial infections.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据