期刊
JOURNAL OF DEVELOPMENTAL ORIGINS OF HEALTH AND DISEASE
卷 13, 期 5, 页码 593-605出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S2040174421000702
关键词
Assisted reproductive technologies; in vitro embryo production; reproductive fluids; growth; hematological parameters; glucose tolerance
资金
- European Union, Horizon 2020 Marie Sklodowska-Curie Action [Rep-Biotech 675526]
- Spanish Ministry of Economy and Competitiveness (MINECO) [AGL2015-66341-R]
- European Regional Development Fund (FEDER) [AGL2015-66341-R]
- Fundacion Seneca, Agencia de Ciencia y Tecnologia de la Region de Murcia [20,040/GERM/16]
- MCIN/AEI [IthornDthorni PID2020-113366RB-I00]
- FEDER Una manera de hacer Europa [PID2020-113366RB-I00]
This study aimed to investigate the long-term effects of adding reproductive fluids (RF) to culture media on piglet phenotype. The results showed that animals derived from in vitro fertilization and embryo culture had larger size at birth and faster growth compared to those from artificial insemination. However, this difference was diminished by the addition of RFs to the culture media. Similarly, small differences in hematological indices and glucose tolerance between animals derived from artificial insemination and in vitro fertilization tended to fade in the presence of RF.
The addition of reproductive fluids (RF) to the culture media has shown benefits in different embryonic traits but its long-term effects on the offspring phenotype are still unknown. We aimed to describe such effects in pigs. Blood samples and growth parameters were collected from piglets derived from in vitro-produced embryos (IVP) with or without RF added in the culture media versus those artificially inseminated (AI), from day 0 to month 6 of life. An oral glucose tolerance test was performed on day 45 of life. We show here the first comparative data of the growth of animals produced through different assisted reproductive techniques, demonstrating differences between groups. Overall, there was a tendency to have a larger size at birth and faster growth in animals derived from in vitro fertilization and embryo culture versus AI, although this trend was diminished by the addition of RFs to the culture media. Similarly, small differences in hematological indices and glucose tolerance between animals derived from AI and those derived from IVP, with a sex-dependent effect, tended to fade in the presence of RF. The addition of RF to the culture media could contribute to minimizing the phenotypical differences between the in vitro-derived and AI offspring, particularly in males.
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