SPOCK1 promotes metastasis in pancreatic cancer via NF-κB-dependent epithelial-mesenchymal transition by interacting with IκB-α

Background Sparc/osteonectin, cwcv and kazal-like domain proteoglycan 1 (SPOCK1) has been reported to function as an oncogene in a variety of cancer types. Increasing evidence suggests that SPOCK1 contributes to the metastatic cascade, including invasion, epithelial-mesenchymal transition (EMT) and micro-metastasis formation. As yet, however, the underlying mechanism is not clearly understood. Here, we evaluated the expression and clinicopathological significance of SPOCK1 in primary pancreatic cancer (PC) specimens and explored the mechanisms underlying SPOCK1-mediated PC cell growth and metastasis. Methods The clinical relevance of SPOCK1 was evaluated in 81 patients with PC. The effect of SPOCK1 on proliferation, cell cycle progression, EMT and metastasis was examined in vitro and in vivo. The molecular mechanisms involved in SPOCK1-mediated regulation of NF-kappa B-dependent EMT were assessed in PC cell lines. Results We found that SPOCK1 expression was increased in PC tissues and was associated with lymph node metastasis. Silencing or exogenous overexpression of SPOCK1 markedly altered the proliferation of PC cells through cell cycle transition. Overexpression of SPOCK1 promoted PC cell migration and invasion by regulating EMT progression. Moreover, we found that SPOCK1 contributes to EMT and metastasis by activating the NF-kappa B signalling pathway via direct interaction with I kappa B alpha. After NF-kappa B pathway inhibition by BAY11-7082, we found that PC cell motility and EMT induced by SPOCK1 were reversed. Conclusion From our data we conclude that SPOCK1 promotes PC metastasis via NF-kappa B-dependent EMT by interacting with I kappa B alpha. This newly identified mechanism may provide novel clues for the (targeted) treatment of PC patients.

Automated summary

SPOCK1 is upregulated in pancreatic cancer tissues and associated with lymph node metastasis. It promotes pancreatic cancer cell proliferation by affecting cell cycle transition and enhances cell migration and invasion by regulating EMT progression. SPOCK1 also activates the NF-kappa B pathway by interacting with I kappa B alpha, leading to EMT and metastasis in pancreatic cancer.