Cytotoxicity analysis of oxazine 4-perchlorate fluorescence nerve potential clinical biomarker for guided surgery

Biological tissue discrimination is relevant in guided surgery. Nerve identification is critical to avoid potentially severe collateral damage. Fluorescence imaging by oxazine 4-perchlorate (O4P) has been recently proposed. In this work, the cytotoxicity of O4P on U87 human-derived glioma cells has been investigated as a function of concentration and operating room irradiation modes. A custom-built optical irradiation device was employed for controlled optical dosimetry. DNA damage and O4P intracellular localization was also investigated by immunofluorescence and confocal microscopy. The results show that concentration below 100 mu M can be considered safe. These results contribute to the assessment of the feasibility of O4P as a nerve biomarker. (c) 2021 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement

Automated summary

This study investigated the cytotoxicity of oxazine 4-perchlorate (O4P) on U87 glioma cells at different concentrations and operating room irradiation modes. DNA damage and intracellular localization of O4P were also studied using immunofluorescence and confocal microscopy. The results suggest that concentrations below 100 μM can be considered safe, contributing to the evaluation of O4P as a nerve biomarker.