期刊
NATURE COMMUNICATIONS
卷 13, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-022-27971-z
关键词
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资金
- National Key R&D Program of China [2019YFC1711008]
- National Natural Science Foundation of China [81773842]
- Ministry of Education, Culture, Sports, Science and Technology, Japan [JP19H04649, 20K16025]
- Grants-in-Aid for Scientific Research [20K16025] Funding Source: KAKEN
2-(2-Phenylethyl)chromones (PECs) are the principal constituents contributing to the distinctive fragrance of agarwood. In this study, the authors identify PECPS, a key enzyme involved in PECs biosynthesis, and provide insight into its catalytic mechanism.
2-(2-Phenylethyl)chromones (PECs) are the principal constituents contributing to the distinctive fragrance of agarwood. How PECs are biosynthesized is currently unknown. In this work, we describe a diarylpentanoid-producing polyketide synthase (PECPS) identified from Aquilaria sinensis. Through biotransformation experiments using fluorine-labeled substrate, transient expression of PECPS in Nicotiana benthamiana, and knockdown of PECPS expression in A. sinensis calli, we demonstrate that the C-6-C-5-C-6 scaffold of diarylpentanoid is the common precursor of PECs, and PECPS plays a crucial role in PECs biosynthesis. Crystal structure (1.98 angstrom) analyses and site-directed mutagenesis reveal that, due to its small active site cavity (247 angstrom(3)), PECPS employs a one-pot formation mechanism including a diketide-CoA intermediate-released step for the formation of the C-6-C-5-C-6 scaffold. The identification of PECPS, the pivotal enzyme of PECs biosynthesis, provides insight into not only the feasibility of overproduction of pharmaceutically important PECs using metabolic engineering approaches, but also further exploration of how agarwood is formed. 2-(2-Phenylethyl)chromones (PECs) contribute to the distinctive fragrance of agarwood. Here the authors identify a diarylpentanoid-producing polyketide synthase from Aquilaria sinensis and show how it catalyzes PEC formation.
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