Circadian neurons in the paraventricular nucleus entrain and sustain daily rhythms in glucocorticoids

It is unclear how circadian signals from the suprachiasmatic nucleus (SCN) are decoded to generate daily rhythms in hormone release. Here, the authors show that daily corticosterone release depends on coordinated clock gene and neuronal activity rhythms in both SCN and paraventricular nucleus neurons. Signals from the central circadian pacemaker, the suprachiasmatic nucleus (SCN), must be decoded to generate daily rhythms in hormone release. Here, we hypothesized that the SCN entrains rhythms in the paraventricular nucleus (PVN) to time the daily release of corticosterone. In vivo recording revealed a critical circuit from SCN vasoactive intestinal peptide (SCNVIP)-producing neurons to PVN corticotropin-releasing hormone (PVNCRH)-producing neurons. PVNCRH neurons peak in clock gene expression around midday and in calcium activity about three hours later. Loss of the clock gene Bmal1 in CRH neurons results in arrhythmic PVNCRH calcium activity and dramatically reduces the amplitude and precision of daily corticosterone release. SCNVIP activation reduces (and inactivation increases) corticosterone release and PVNCRH calcium activity, and daily SCNVIP activation entrains PVN clock gene rhythms by inhibiting PVNCRH neurons. We conclude that daily corticosterone release depends on coordinated clock gene and neuronal activity rhythms in both SCNVIP and PVNCRH neurons.

Automated summary

The daily release of corticosterone depends on coordinated clock gene and neuronal activity rhythms in both suprachiasmatic nucleus vasoactive intestinal peptide (SCNVIP) and paraventricular nucleus corticotropin-releasing hormone (PVNCRH) neurons. The signals from the central circadian pacemaker, the suprachiasmatic nucleus (SCN), are decoded to generate daily rhythms in hormone release through an SCN- PVN circuit. Loss of the clock gene Bmal1 in CRH neurons results in arrhythmic PVNCRH calcium activity and dramatically reduces the amplitude and precision of daily corticosterone release.