Bacterial filamentation as a mechanism for cell-to-cell spread within an animal host

Some intracellular pathogens can directly invade neighboring host cells in cell culture, but it is unclear how this happens in vivo. Here, Tran et al. describe an intracellular bacterium that forms filaments to spread between intestinal epithelial cells in its host nematode, in a process regulated by a conserved nutrient-sensing pathway. Intracellular pathogens are challenged with limited space and resources while replicating in a single host cell. Mechanisms for direct invasion of neighboring host cells have been discovered in cell culture, but we lack an understanding of how bacteria directly spread between host cells in vivo. Here, we describe the discovery of intracellular bacteria that use filamentation for spreading between the intestinal epithelial cells of a natural host, the rhabditid nematode Oscheius tipulae. The bacteria, which belong to the new species Bordetella atropi, can infect the nematodes following a fecal-oral route, and reduce host life span and fecundity. Filamentation requires UDP-glucose biosynthesis and sensing, a highly conserved pathway that is used by other bacteria to detect rich conditions and inhibit cell division. Our results indicate that B. atropi uses a pathway that normally regulates bacterial cell size to trigger filamentation inside host cells, thus facilitating cell-to-cell dissemination.

Automated summary

The study reveals the mechanism by which an intracellular bacterium spreads between intestinal epithelial cells of a natural host by forming filaments. This process is regulated by a conserved nutrient-sensing pathway and reduces host lifespan and fecundity.