期刊
NATURE COMMUNICATIONS
卷 13, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-27895-0
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资金
- Cambridge NIHR BRC Cell Phenotyping Hub
- Engineering and Physical Sciences Research Council [EP/P020259/1]
- Science and Technology Facilities Council
- ISRHML trainee bridge fund postdoctoral fellowship
- Helmholtz Postdoctoral Fellowship
- Cambridge Cancer Centre PhD studentship
- UKRI-MRC project [MR/S036059/1]
- UKRI-BBSRC project [BB/S006745/1]
- Breast Cancer Now Project [2017MayPR907]
- CRUK Programme Foundation Award [DCRPGF\100010]
This study characterizes cells in human milk and identifies epithelial cells similar to luminal progenitors and immune cells. The findings contribute to understanding the remodelling of human mammary tissue during pregnancy and lactation, and provide insights into the interplay between pregnancy, lactation, and breast cancer.
Human mammary tissue remodelling that takes place during pregnancy and lactation remains poorly understood. Here the authors characterize cells in human milk, identifying epithelial cells resembling luminal progenitors and immune cells, contributing insights into this process. Under normal conditions, the most significant expansion and differentiation of the adult mammary gland occurs in response to systemic reproductive hormones during pregnancy and lactation to enable milk synthesis and secretion to sustain the offspring. However, human mammary tissue remodelling that takes place during pregnancy and lactation remains poorly understood due to the challenge of acquiring samples. We report here single-cell transcriptomic analysis of 110,744 viable breast cells isolated from human milk or non-lactating breast tissue, isolated from nine and seven donors, respectively. We found that human milk largely contains epithelial cells belonging to the luminal lineage and a repertoire of immune cells. Further transcriptomic analysis of the milk cells identified two distinct secretory cell types that shared similarities with luminal progenitors, but no populations comparable to hormone-responsive cells. Taken together, our data offers a reference map and a window into the cellular dynamics that occur during human lactation and may provide further insights on the interplay between pregnancy, lactation and breast cancer.
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