4.8 Article

High-throughput identification and quantification of single bacterial cells in the microbiota

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16S rRNA Gene Copy Number Normalization Does Not Provide More Reliable Conclusions in Metataxonomic Surveys

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Summary: Sequencing 16S rRNA gene amplicons is commonly used to study prokaryotic communities, but gene copy normalization (GCN) does not improve the accuracy of results. The study questions the use of GCN for metataxonomic surveys until a more comprehensive catalogue of copy numbers becomes available.

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