期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-27201-y
关键词
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资金
- SAMANA Kay MGH Research Scholars award
- Ragon Institute of MGH, MIT, and Harvard
- Massachusetts Consortium on Pathogen Readiness (MassCPR)
- NIH [3R37AI080289-11S1, R01AI146785, R01-AI132633, U19AI42790-01, U19AI135995-02, 1U01CA260476 - 01, CIVIC75N93019C00052, T32 AI007061, R01AI123654, R01AI143453, 3UL1TR002556-04S1]
- G. Harold and Leila Y. Mathers Foundation
- Gates Foundation
- Global Health Vaccine Accelerator Platform funding [OPP1146996, INV-001650]
- Musk Foundation
- Price Family Foundation
The study provides insights into the significant impact of convalescent plasma on modulating humoral responses by focusing on spike/nucleocapsid in recipients.
Transfer of convalescent plasma (CP) had been proposed early during the SARS-CoV-2 pandemic as an accessible therapy, yet trial results worldwide have been mixed, potentially due to the heterogeneous nature of CP. Here we perform deep profiling of SARS-CoV-2-specific antibody titer, Fc-receptor binding, and Fc-mediated functional assays in CP units, as well as in plasma from hospitalized COVID-19 patients before and after CP administration. The profiling results show that, although all recipients exhibit expanded SARS-CoV-2-specific humoral immune responses, CP units contain more functional antibodies than recipient plasma. Meanwhile, CP functional profiles influence the evolution of recipient humoral immunity in conjuncture with the recipient's pre-existing SARS-CoV2-specific antibody titers: CP-derived SARS-CoV-2 nucleocapsid-specific antibody functions are associated with muted humoral immune evolution in patients with high titer anti-spike IgG. Our data thus provide insights into the unexpected impact of CP-derived functional anti-spike and anti-nucleocapsid antibodies on the evolution of SARS-CoV-2-specific response following severe infection. Convalescent plasma (CP) has been trialed as a therapy for SARS-CoV-2 symptoms, but its heterogenous nature precludes uniform outcomes. Here the authors perform deep profiling of CP, as well as plasma of CP recipients before and after transfer, to find CP-mediated, spike/nucleocapsid-focused modulations of humoral responses in the recipient.
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