期刊
NATURE COMMUNICATIONS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41467-021-26737-3
关键词
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资金
- NARSAD Young Investigator Grant from the Brain & Behavior Research Foundation
- Richard and Susan Smith Family Foundation
- Elizabeth and Stuart Pratt Career Development Award
- Whitehall Foundation
- National Science Foundation Neuronex Neurotechnology Hub (NEMONIC) [1707287]
- National Institutes of Health BRAIN Initiative Award [UF1NS107705]
- National Institutes of Health New Innovator Award [DP2NS111134]
Understanding brain function requires monitoring both local and global brain dynamics, with two-photon imaging presenting trade-offs between imaging area and acquisition speed. The authors introduce Quadroscope, a flexible microscope capable of simultaneously capturing four independently targetable brain regions at video rate across 5 mm field of view, demonstrating the ability to measure calcium activity in the mouse sensorimotor cortex at behaviorally relevant timescales.
Understanding brain function requires monitoring local and global brain dynamics. Two-photon imaging of the brain across mesoscopic scales has presented trade-offs between imaging area and acquisition speed. We describe a flexible cellular resolution two-photon microscope capable of simultaneous video rate acquisition of four independently targetable brain regions spanning an approximate five-millimeter field of view. With this system, we demonstrate the ability to measure calcium activity across mouse sensorimotor cortex at behaviorally relevant timescales. Functional brain imaging with two-photon microscopy is limited by a tradeoff between imaging area and acquisition speed. Here, the authors present Quadroscope, a flexible microscope which allows for simultaneous video rate acquisition of four independently targetable brain regions across 5 mm.
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