期刊
ONCOLOGY LETTERS
卷 23, 期 4, 页码 -出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2022.13230
关键词
cervical cancer; matrine; autophagy; Akt; mTOR signaling pathway
类别
资金
- Shaanxi Provincial Key RD Program [2017ZDXM-SF-26-3]
- National Natural Science Foundation of China [81702578]
This study showed that matrine can inhibit the proliferation of cervical cancer cells and induce autophagy by inhibiting the Akt/mTOR signaling pathway. Additionally, the combination of matrine and cisplatin was more effective in suppressing the proliferation of cervical cancer cells both in vitro and in vivo.
Matrine is a quinazoline alkaloid extracted from Sophora flavescens. The aim of the present study was to determine whether matrine can induce autophagy in the human HeLa and SiHa cervical cancer cell lines in vitro and in vivo. Cell viability assay was used to assess the suppressive effect of matrine and cisplatin on the proliferation of HeLa and SiHa cells. A total of 28 4-week-old female BALB/c nude mice were used for the in vivo study. Autophagy and protein expression were observed via transmission electron microscopy, monodansylcadaverine and immunohistochemical staining and western blotting. The inhibitory effect of matrine on the proliferation of cervical cancer cells was time- and dose-dependent. The combination of matrine and cisplatin synergistically inhibited the proliferation of cervical cancer cells in vitro and in vivo. Transmission electron microscopy showed that after the addition of matrine, numerous autophagosomes and autophagolysosomes were observable in HeLa and SiHa cells, as demonstrated by monodansylcadaverine staining. Western blotting and immunohistochemical staining showed that as the concentration of matrine increased, the expression of the autophagy marker LC3A/B-II also increased significantly in vitro and in vivo. These findings suggested that matrine inhibited the proliferation of cervical cancer cells and induced autophagy by inhibiting the Akt/mTOR signaling pathway. Thus, matrine may represented a potential candidate in combination therapy for cervical cancer as an inducer of autophagy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据