Inhibition of Aspergillus flavus growth and aflatoxin B1 production by natamycin

Aspergillus flavus causes huge crop losses, reduces crop quality and has adverse effects on human and animal health. A large amount of food contaminated with aflatoxin can greatly increase the risk of liver cancer. Therefore, prevention and control of aflatoxin production have aroused attention of research in various countries. Natamycin extracted from Streptomyces spp. has been widely used in production practice due to its good specificity and safety. Here, we found that natamycin could significantly inhibit fungal growth, conidia germination, ergosterol and AFB 1 production by A.flavus in a dose-dependent manner. Scanning electron microscope analysis indicated that the number of conidia was decreased, the outer wall of conidia was destroyed, and the mycelia were shrivelled and tangled by natamycin. RNA-Seq data indicated that natamycin inhibited fungal growth and conidia development of A. flavus by significantly down-regulating some genes involved in ergosterol biosynthesis, such as Erg13, HMG1 and HMG2. It inhibited conidia germination by significantly down-regulating some genes related to conidia development, such as FluG and VosA. After natamycin exposure, the decreased ratio of aflS/aflR caused by the down-regulation of all the structural genes, which subsequently resulted in the suppression of AFB(1) production. In conclusion, this study served to reveal the inhibitory mechanisms of natamycin on fungal growth and AFB(1) biosynthesis in A. flavus and to provide solid evidence for its application in controlling AFB(1) contamination.

Automated summary

Aspergillus flavus is a pathogenic fungus that causes significant crop losses and has negative effects on human and animal health. This study found that natamycin, extracted from Streptomyces spp., can effectively inhibit fungal growth and conidia germination, as well as reduce the production of ergosterol and AFB 1 by A. flavus. The inhibitory effects of natamycin on fungal growth and AFB 1 biosynthesis were achieved by down-regulating genes involved in ergosterol biosynthesis and conidia development. The findings provide solid evidence for the application of natamycin in controlling AFB 1 contamination.