From bulk, single-cell to spatial RNA sequencing

RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA sequencing (RNAseq), popular single cell RNA sequencing (scRNAseq) to newly emerged spatial RNA sequencing (spRNAseq). Bulk RNAseq studies average global gene expression, scRNAseq investigates single cell RNA biology up to 20,000 individual cells simultaneously, while spRNAseq has ability to dissect RNA activities spatially, representing next generation of RNA sequencing. This article highlights these technologies, characteristic features and suitable applications in precision oncology.

Automated summary

The evolution of RNA sequencing technology has enhanced the completeness of genetic information, with bulk RNAseq averaging global gene expression, scRNAseq investigating single-cell RNA biology, and spRNAseq dissecting RNA activities spatially, representing the next generation of RNA sequencing.