期刊
CELL DEATH & DISEASE
卷 12, 期 10, 页码 -出版社
SPRINGERNATURE
DOI: 10.1038/s41419-021-04189-3
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资金
- Ligue Contre le Cancer Midi-Pyrenees
- ANR PRC-2019
- Ligue Nationale Contre le Cancer (Labellisation 2018)
Centromeres, defined by chromatin containing CENP-A, play a crucial role in chromosome inheritance and genome stability. BRCA1 associates with centromeres to counteract R-loop accumulation at centromeric alpha-satellite repeats, maintaining centromere stability and identity.
Centromeres are defined by chromatin containing the histone H3 variant CENP-A assembled onto repetitive alpha-satellite sequences, which are actively transcribed throughout the cell cycle. Centromeres play an essential role in chromosome inheritance and genome stability through coordinating kinetochores assembly during mitosis. Structural and functional alterations of the centromeres cause aneuploidy and chromosome aberrations which can induce cell death. In human cells, the tumor suppressor BRCA1 associates with centromeric chromatin in the absence of exogenous damage. While we previously reported that BRCA1 contributes to proper centromere homeostasis, the mechanism underlying its centromeric function and recruitment was not fully understood. Here, we show that BRCA1 association with centromeric chromatin depends on the presence of R-loops, which are non-canonical three-stranded structures harboring a DNA:RNA hybrid and are frequently formed during transcription. Subsequently, BRCA1 counteracts the accumulation of R-loops at centromeric alpha-satellite repeats. Strikingly, BRCA1-deficient cells show impaired localization of CENP-A, higher transcription of centromeric RNA, increased breakage at centromeres and formation of acentric micronuclei, all these features being R-loop-dependent. Finally, BRCA1 depletion reveals a Rad52-dependent hyper-recombination process between centromeric satellite repeats, associated with centromere instability and missegregation. Altogether, our findings provide molecular insights into the key function of BRCA1 in maintaining centromere stability and identity.
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