期刊
JOURNAL OF FUNCTIONAL FOODS
卷 86, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jff.2021.104715
关键词
Bifidobacterium longum K2-21-4; CCD841 CoN; Tight junction; Proliferation
资金
- Natural Science Foundation of Heilongjiang Province [YQ2020C013]
- National Key Research and Development Program of China [2017YFD0400303]
- National Natural Science Foundation of China [32072190]
- Academic Backbone Plan of Northeast Agricultural University [19YJXG10]
Bifidobacterium longum subsp. longum strain K2-21-4 can promote CCD841 CoN cell proliferation, accelerate the cell cycle process, decrease cell apoptosis, and activate proteins related to tight junctions. These findings suggest that B. longum K2-21-4 can regulate intestinal development by promoting cell proliferation and accelerating the maturation of the internal barrier.
Bifidobacterium species are the dominant bacteria in the intestinal tract of infants and young children, and are closely related to intestinal development. The Bifidobacterium longum subsp. longum strains K2-21-4 has been shown to promote the proliferation of CCD841 CoN cells. We evaluate the effect of B. longum on the proliferation of CCD841 CoN cells and tight junction using a lipopolysaccharide (LPS)-induced injury model. We discovered that B. longum K2-21-4 increased the cell proliferation rate and total cell protein content, shortened the cell cycle process, reduced apoptosis, and increased the activity of adenosine triphosphate (ATP)ases, aspartate aminotransferase, and alanine aminotransferase. Real-time reverse transcription quantitative polymerase chain reaction and western blotting showed that B. longum K2-21-4 activated Wnt/beta-Catenin and PI3K/Akt/mTOR signaling pathway at gene transcription and protein expression levels up-regulated the expression level of protein related to the tight junction. Our findings suggest that B. longum K2-21-4 could regulate intestinal development by promoting cell proliferation and accelerating the maturation of the internal barrier.
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