期刊
FRONTIERS IN CELLULAR NEUROSCIENCE
卷 15, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fncel.2021.814547
关键词
arrestin; biosensor; cannabinoid; dynamics; G protein coupled receptor (GPCR); kinetics; opioid; partial agonist
资金
- National Institute of General Medical Sciences, National Institutes of Health [R44GM125390]
- National Institute on Drug Abuse, National Institutes of Health [R44NS082222]
- National Health and Medical Research Council [1107088]
- National Institutes of Health [P01DA009158]
- European Union [FP7/2007-103, HEALTH-F2-2011-278850, R21DA045882]
- National Health and Medical Research Council of Australia [1107088] Funding Source: NHMRC
Neurons integrate inputs over different time and space scales, combining fast and slow signals to produce behavior. Measuring signaling kinetics in live cells using fluorescent biosensors and dyes provides a deeper understanding of G-protein-coupled receptor signaling and therapeutic mechanisms in the nervous system.
Neurons integrate inputs over different time and space scales. Fast excitatory synapses at boutons (ms and mu m), and slow modulation over entire dendritic arbors (seconds and mm) are all ultimately combined to produce behavior. Understanding the timing of signaling events mediated by G-protein-coupled receptors is necessary to elucidate the mechanism of action of therapeutics targeting the nervous system. Measuring signaling kinetics in live cells has been transformed by the adoption of fluorescent biosensors and dyes that convert biological signals into optical signals that are conveniently recorded by microscopic imaging or by fluorescence plate readers. Quantifying the timing of signaling has now become routine with the application of equations in familiar curve fitting software to estimate the rates of signaling from the waveform. Here we describe examples of the application of these methods, including (1) Kinetic analysis of opioid signaling dynamics and partial agonism measured using cAMP and arrestin biosensors; (2) Quantifying the signaling activity of illicit synthetic cannabinoid receptor agonists measured using a fluorescent membrane potential dye; (3) Demonstration of multiplicity of arrestin functions from analysis of biosensor waveforms and quantification of the rates of these processes. These examples show how temporal analysis provides additional dimensions to enhance the understanding of GPCR signaling and therapeutic mechanisms in the nervous system.
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