期刊
BIOFABRICATION
卷 14, 期 2, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/1758-5090/ac4fb6
关键词
3D cell-printing; tissue-specific bioink; in vitro preconditioning; biomimetic tissue construct; tendon and ligament regeneration
资金
- National Research Foundation of Korea (NRF) - Korea Government (MSIP) [2020M3H4A1A02084827]
- Fundamental Research Program of the Korea Institute of Materials Science [PNK8170]
- National Research Council of Science & Technology (NST), Republic of Korea [PNK8170] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study presents a cell-based tissue engineering strategy using 3D cell-printing technology to generate cell-laden tissue constructs for tendon and ligament regeneration. The implementation of an in vitro preconditioning approach promotes tissue formation and improves cell survival and retention upon in vivo implantation. These findings have important implications for future cell-based therapies in tendon and ligament regeneration.
Tendon and ligament tissues provide stability and mobility crucial for musculoskeletal function, but are particularly prone to injury. Owing to poor innate healing capacity, the regeneration of mature and functional tendon/ligament (T/L) poses a formidable clinical challenge. Advanced bioengineering strategies to develop biomimetic tissue implants are highly desired for the treatment of T/L injuries. Here, we presented a cell-based tissue engineering strategy to generate cell-laden tissue constructs comprising stem cells and tissue-specific bioinks using 3D cell-printing technology. We implemented an in vitro preconditioning approach to guide semi-organized T/L-like formation before the in vivo application of cell-printed implants. During in vitro maturation, tissue-specific decellularized extracellular matrix-based cellular constructs facilitated long-term in vitro culture with high cell viability and promoted tenogenesis with enhanced cellular/structural anisotropy. Moreover, we demonstrated improved cell survival/retention upon in vivo implantation of pre-matured constructs in nude mice with de novo tendon formation and improved mechanical strength. Although in vivo mechanical properties of the cell-printed implants were lower than those of human T/L tissues, the results of this study may have significant implications for future cell-based therapies in tendon and ligament regeneration and translational medicine.
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