期刊
BIOFABRICATION
卷 14, 期 3, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/1758-5090/ac57a6
关键词
carbon nanotubes; brain-derived neurotrophic factor; polydopamine; electrospinning; peripheral nerve regeneration
资金
- National Natural Science Foundation of China [31771322]
- Beijing Natural Science Foundation of China [7212121]
- Shenzhen Science and Technology Plan Project of China [JCYJ20190806162205278]
This study fabricated aligned conductive scaffolds composed of carbon nanotubes (CNTs) via electrospinning and loaded brain-derived neurotrophic factor (BDNF) onto the scaffolds. The results showed that the scaffolds effectively promoted peripheral nerve regeneration both in vitro and in vivo, indicating their potential for nerve restoration.
Carbon nanotubes (CNTs) have attracted increasing attention in the field of peripheral nerve tissue engineering due to their unique structural and physical characteristics. In this study, a novel type of aligned conductive scaffolds composed of polycaprolactone (PCL) and CNTs were fabricated via electrospinning. Utilizing mussel-inspired polydopamine (PDA) surface modification, brain-derived neurotrophic factor (BDNF) was loaded onto PCL/CNT fibrous scaffolds to obtain PCL/CNT-PDA-BDNF fibrous scaffolds capable of the sustained release of BDNF over 28 d. Schwann cells were cultured on these scaffolds, and the effect of the scaffolds on peripheral nerve regeneration in vitro was assessed by studying cell proliferation, morphology and the expressions of myelination-related genes S100, P0 and myelin basic protein. Furthermore, the effect of these scaffolds on peripheral nerve regeneration in vivo was investigated using a 10 mm rat sciatic nerve defect model. Both the in vitro and in vivo results indicate that PCL/CNT-PDA-BDNF fibrous scaffolds effectively promote sciatic nerve regeneration and functional recovery. Therefore, PCL/CNT-PDA-BDNF fibrous scaffolds have great potential for peripheral nerve restoration.
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