期刊
BIOFABRICATION
卷 14, 期 1, 页码 -出版社
IOP Publishing Ltd
DOI: 10.1088/1758-5090/ac2f81
关键词
cell-assembled extracellular matrix; bioprinting; laser microdissection; vasculogenesis
资金
- 'L'Institut national de la sante et de la recherche medicale' Inserm, France
- 'Plateforme de microdissection laser', Neurocentre Magendie, Bordeaux, France
This study emphasizes the importance of creating a functional vascular system for the survival of bioengineered tissues. The authors experimented with a new biomaterial called cell-assembled extracellular matrix (CAM) to create a vascular system. They demonstrated successful production and maturation of capillary-like structures using CAM sheets, which were able to connect to the host circulatory system in a mouse model.
When considering regenerative approaches, the efficient creation of a functional vasculature, that can support the metabolic needs of bioengineered tissues, is essential for their survival after implantation. However, it is widely recognized that the post-implantation microenvironment of the engineered tissues is often hypoxic due to insufficient vascularization, resulting in ischemia injury and necrosis. This is one of the main limitations of current tissue engineering applications aiming at replacing significant tissue volumes. Here, we have explored the use of a new biomaterial, the cell-assembled extracellular matrix (CAM), as a biopaper to biofabricate a vascular system. CAM sheets are a unique, fully biological and fully human material that has already shown stable long-term implantation in humans. We demonstrated, for the first time, the use of this unprocessed human ECM as a microperforated biopaper. Using microvalve dispensing bioprinting, concentrated human endothelial cells (30 millions ml(-1)) were deposited in a controlled geometry in CAM sheets and cocultured with HSFs. Following multilayer assembly, thick ECM-based constructs fused and supported the survival and maturation of capillary-like structures for up to 26 d of culture. Following 3 weeks of subcutaneous implantation in a mice model, constructs showed limited degradative response and the pre-formed vasculature successfully connected with the host circulatory system to establish active perfusion.This mechanically resilient tissue equivalent has great potential for the creation of more complex implantable tissues, where rapid anastomosis is sine qua non for cell survival and efficient tissue integration.
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