Rapid Biosensor of SARS-CoV-2 Using Specific Monoclonal Antibodies Recognizing Conserved Nucleocapsid Protein Epitopes

Article Infectious Diseases

Accuracy of a nucleocapsid protein antigen rapid test in the diagnosis of SARS-CoV-2 infection

Bo Diao et al.

Summary: The FIC assay for NP antigen testing shows high specificity and relatively high sensitivity in early-phase SARS-CoV-2 diagnosis, according to a study conducted in Wuhan and Chongqing between February 10 and 15, 2020.

CLINICAL MICROBIOLOGY AND INFECTION (2021)

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Article Virology

Evolutionary dynamics of SARS-CoV-2 nucleocapsid protein and its consequences

M. Shaminur Rahman et al.

Summary: The study characterized mutations in the nucleocapsid (N) protein of SARS-CoV-2, identifying a significant number of nucleotide and amino acid mutations. Particularly noteworthy were mutations at primer binding sites and high-frequency mutations impacting the protein's structure.

JOURNAL OF MEDICAL VIROLOGY (2021)

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Article Biophysics

A novel rapid detection for SARS-CoV-2 spike 1 antigens using human angiotensin converting enzyme 2 (ACE2)

Jong-Hwan Lee et al.

Summary: This study presents a novel rapid detection method for the SARS-CoV-2 S1 antigen using a matched pair of ACE2 and antibody. The detection method showed high specificity and sensitivity without cross-reactivity, making it a valuable tool in detecting the S1 antigen from COVID-19 patients.

BIOSENSORS & BIOELECTRONICS (2021)

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Review Biochemistry & Molecular Biology

COVID-19 vaccines: where we stand and challenges ahead

Guido Forni et al.

Summary: The development of vaccines has made significant progress, but still faces various challenges, including different target populations, immunological adaptability of vaccines, and production and distribution issues. To ensure equitable access, protection of diverse subjects, and immunity against viral variants, multiple vaccines may be needed in the long run.

CELL DEATH AND DIFFERENTIATION (2021)

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Article Biophysics

Development of a SARS-CoV-2-specific biosensor for antigen detection using scFv-Fc fusion proteins

Hye-Yeon Kim et al.

Summary: This study presents the development of a specific LFIA-based biosensor for COVID-19 using phage display technology to generate antibodies targeting SARS-CoV-2 NP antigen, allowing rapid and specific detection of the virus. The newly developed rapid diagnostic test shows potential for use in the diagnosis of COVID-19.

BIOSENSORS & BIOELECTRONICS (2021)

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Letter Biotechnology & Applied Microbiology

Could mutations of SARS-CoV-2 suppress diagnostic detection?

Carl A. Ascoli

NATURE BIOTECHNOLOGY (2021)

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Article Multidisciplinary Sciences

SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity

Seiya Ozono et al.

Summary: The naturally occurring mutations of SARS-CoV-2 spike protein can affect cell entry, with the D614G mutation resulting in increased cell entry and maintaining susceptibility to neutralization by antisera against prototypic viruses. Further global surveillance is needed to understand the transmissibility of SARS-CoV-2 among humans.

NATURE COMMUNICATIONS (2021)

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Review Immunology

SARS-CoV-2 vaccines strategies: a comprehensive review of phase 3 candidates

Nikolaos C. Kyriakidis et al.

Summary: The new SARS-CoV-2 virus is rapidly spreading worldwide, causing the COVID-19 pandemic with more than 1.866 million deaths. Over 64 vaccine candidates are being developed globally, aiming to induce antibodies to prevent viral entry. Thirteen vaccine candidates are currently in Phase 3 clinical trials, showing promising progress towards approval for large-scale immunizations.

NPJ VACCINES (2021)

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Review Biotechnology & Applied Microbiology

COVID-19 in-vitro Diagnostics: State-of-the-Art and Challenges for Rapid, Scalable, and High-Accuracy Screening

Zeina Habli et al.

Summary: The global struggle with the COVID-19 pandemic has led to the development of over 140 diagnostic assays, but uncertainties remain regarding the performance and accuracy of these testing modalities.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2021)

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Review Microbiology

SARS-CoV-2 variants, spike mutations and immune escape

William T. Harvey et al.

Summary: The evolution of SARS-CoV-2 has been characterized by the emergence of mutations and variants that impact virus characteristics. Manufacturers are preparing for possible updates to vaccines in response to changes in the virus population, and it is crucial to monitor genetic and antigenic changes alongside experiments to understand the impacts of mutations.

NATURE REVIEWS MICROBIOLOGY (2021)

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Review Virology

The SARS-CoV-2 Nucleocapsid Protein and Its Role in Viral Structure, Biological Functions, and a Potential Target for Drug or Vaccine Mitigation

Zhihua Bai et al.

Summary: The N protein of SARS-CoV-2 shares high homology with other coronavirus N proteins, indicating its potential significance as a drug target or vaccine candidate. Further research is needed to explore the functional aspects of this protein in controlling the spread of the virus.

VIRUSES-BASEL (2021)

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Article Cell Biology

Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests

Yutaro Yamaoka et al.

Summary: This study produced monoclonal antibodies that exclusively react with SARS-CoV-2 with no cross-reactivity with other human coronaviruses, suitable for detecting the virus in clinical samples. The antibodies were selected and used to develop an immuno-chromatographic assay augmented with silver amplification technology, capable of detecting current circulating variants of concern. These antibodies can serve as good candidates for developing antigen detection kits for COVID-19 due to their high performance.

CELL REPORTS MEDICINE (2021)

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Article Medicine, General & Internal