期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 35, 期 -, 页码 77-84出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.intimp.2016.03.029
关键词
Rutin; UUO; Tubulointerstitial fibrosis; Inflammation
资金
- National Natural Science Foundation of China [81170696]
Rutin reportedly conveys many beneficial effects, including renoprotection; however, it has not yet been demonstrated to have a renoprotective effect against obstructive nephropathy. The present study is the first to show a protective effect of rutin against obstructive renal injury induced by unilateral ureteral obstruction (DUO). A total of 24 male Wistar rats were randomly divided into four groups of six rats each, including vehicle- or rutin-treated sham operated groups, and vehicle- or rutin-treated DUO groups. Rats received daily oral gavage of rutin (100 mg/kg) for 2 weeks. All rats were euthanized on postoperative day 14. Histological findings showed that rutin administration significantly reduced renal interstitial injury and suppressed interstitial collagen deposits in DUO rats. Moreover, rutin decreased macrophage infiltration, proinflammatory cytokine expression and phosphorylation of nuclear factor-kappa B p65. Furthermore, rutin inhibited extracellular matrix accumulation by reducing expression of type I/III collagen and fibronectin. Rutin also prevented the epithelial-mesenchymal transition processes of renal tubular cells by decreasing alpha-smooth muscle actin expression and retaining E-cadherin expression. These effects of rutin were in parallel with the reductions in Smad3 activity and pivotal to the fibrogenic potential of TGF-beta 1. Taken together, the renoprotective effects of rutin in obstructive nephropathy were likely due to anti-inflammatory effects and inhibition of TGF-beta 1/Smad3 signaling. (C) 2016 Elsevier B.V. All rights reserved.
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