期刊
ZEBRAFISH
卷 19, 期 1, 页码 1-6出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/zeb.2021.0075
关键词
teleost; zebrafish; ontogeny; osmoregulation; nano-osmometry
The zebrafish, a widely used model species, has been studied extensively in various research fields. However, little is known about its blood osmolality. In this study, we measured the blood osmolality of zebrafish at different stages of postembryonic development and found significant changes in osmolality during growth and metamorphosis.
The zebrafish Danio rerio is a teleost model species widely used in developmental genetics, biomedical studies, toxicology, and drug screening. Despite the interest of this species in research, little is known through indirect observations about its blood osmolality, which is a key parameter for diverse experiments. In this study, we directly measured blood osmolality using nano-osmometry at different stages of zebrafish postembryonic development. We found that blood osmolality is close to 240 mOsm center dot kg(-1) in early larvae. It progressively increased to similar to 270 mOsm center dot kg(-1) during the larval development before reaching similar to 300 mOsm center dot kg(-1) after metamorphosis in juveniles and later in adults. These ontogenetic changes in blood osmolality illustrate the physiological changes in osmoregulation associated with postembryonic development, including metamorphosis. These values are of practical interest for adjusting the osmolality of fixatives and cell and tissue culture media for research using zebrafish as a model.
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