Different responses of gram-negative and gram-positive bacteria to photocatalytic disinfection using solar-light-driven magnetic TiO2-based material, and disinfection of real sewage

A solar-light-driven magnetic photocatalyst, reduced-graphene-oxide/Fe,H-TiO2/Fe3O4@SiO2 (RGOFeNTFS), was developed for the photocatalytic disinfection of different strains of bacteria: gram-negative Escherichia coli (E. coli) and Salmonella typhimurium (S. typhimurium), and gram-positive Enterococcus faecalis (E. faecalis). The different responses of the bacteria during the reaction were investigated. Gram-positive E. faecalis was found to be more susceptible to photocatalytic disinfection and exhibited a higher leakage of intracellular components than the two gram-negative bacteria. The interactions between the bacteria and RGOFeNTFS were analyzed for Zeta potential, hydrophilicity and SEM. Under the experimental conditions, the opposite surface charges of the bacteria (negative Zeta potential) and RGOFeNTFS (positive Zeta potential) contribute to their interactions. With a more negative Zeta potential (than E. coli and E. faecalis), S. typhimurium interacts more strongly with RGOFeNTFS and is mainly attacked by center dot OH near the photocatalyst surface. E. coli and E. faecalis (with less negative Zeta potentials) interact less strongly with RGOFeNTFS, and compete for the dominant reactive species (center dot O-2(-)) in the bulk solution. Therefore, the co-existence of bacteria significantly inhibits the photocatalytic disinfection of E. coli and E. faecalis, but insignificantly for S. typhimurium. Moreover, photocatalytic disinfection using RGOFeNTFS show potential for treating real sewage, which meets the local discharge standard (of E. coli) after a 60-min reaction. In real sewage, different bacteria are disinfected simultaneously.

Automated summary

The study demonstrated that gram-positive bacteria were more susceptible to photocatalytic disinfection, showing higher leakage of intracellular components. Gram-negative bacteria interacted less strongly with the photocatalyst, leading to competition for reactive species and inhibition of disinfection effectiveness.