期刊
WASTE MANAGEMENT
卷 135, 期 -, 页码 60-69出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.wasman.2021.08.030
关键词
Polyethylene; Polyhydroxyalkanoate; Bioconversion; High cell density; Fermentation; Pseudomonas putida
资金
- Environmental Protection Agency of Ireland [2008-ET-LS-1-S2]
- Science Foundation Ireland [16/RC/3889]
- Ministry of Science and Higher Education of Poland within the Regional Initiative of Excellence Program for 2019-2022 [021/RID/2018/19]
- Environmental Protection Agency Ireland (EPA) [2008-ET-LS-1-S2] Funding Source: Environmental Protection Agency Ireland (EPA)
The study optimized the production of medium-chain-length polyhydroxyalkanoate (mcl-PHA) using Pseudomonas putida KT2440 fed with a polyethylene (PE)-derived fatty acid mixture. The results showed high mcl-PHA yields in a 20-L setup, indicating the potential environmental advantages of processing waste PE into PHA instead of incineration.
In this study, the optimisation of a process for producing medium-chain-length polyhydroxyalkanoate (mcl-PHA) by Pseudomonas putida KT2440 when fed with a polyethene (PE)-derived fatty acid mixture was investigated. The PE was pyrolysed to produce a hydrocarbon wax that was subsequently oxidised to produce a mixture of fatty acids, purified, and used as a PHA substrate for the growth and selection of microorganisms. Based on the shaken flask screening, a production strain, i.e., Pseudomonas putida KT2440, was selected for conducting bioreactor studies. Feeding PE-derived fatty acids in a 20-L setup resulted in high mcl-PHA yields (83.0 g L-1 CDW with 65% PHA in 25 h). Furthermore, life-cycle assessment (LCA) was conducted to determine the environmental advantages of the proposed process and its impacts compared to those of other technologies for treating PE-derived waste streams. We conclude that processing waste PE into PHA, rather than incineration, produces biodegradable material while also reducing the additional emissions that arise from traditional PE waste treatment processes, such as incineration to gain energy.
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