期刊
VIROLOGY
卷 562, 期 -, 页码 158-175出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2021.07.014
关键词
Abutilon mosaic virus; Geminivirus; Begomovirus; Virus movement; Protein-protein interaction; Forster resonance energy transfer; FRET; Endosomes; Actin; Microtubule
类别
资金
- LGFG grant
The study reveals that the nuclear shuttle protein (NSP) and movement protein (MP) of Abutilon mosaic virus use separate cellular pathways for viral DNA transport in plants, without physical interaction between the two proteins.
The Abutilon mosaic virus (AbMV) encodes a nuclear shuttle protein (NSP), and a movement protein (MP) which cooperatively accomplish viral DNA transport through the plant. Subcellular distribution patterns of fluorescent protein-tagged NSP and MP were tracked in Nicotiana benthamiana leaves in presence or absence of an AbMV infection using light microscopy. NSP was located within the nucleus and associated with early endosomes in the presence of MP. MP appeared at the plasma membrane, plasmodesmata and in motile vesicles, trafficking along the endoplasmic reticulum in an actin-dependent manner. MP and NSP did not co-localize and employed separate cellular pathways. Correspondingly, Forster resonance energy transfer analysis did not support physical interaction between NSP and MP. Time lapse movies illustrate the cellular dynamics of both proteins on their way around the nucleus and to the cell periphery and provide a first hint for the nuclear egress of NSP complexes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据