期刊
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
卷 20, 期 4, 页码 715-720出版社
PHARMACOTHERAPY GROUP
DOI: 10.4314/tjpr.v20i4.8
关键词
Cynaropicrin; Anti-proliferation; Pro-apoptosis; Caspase-3; Artichoke
资金
- Scientific Research Project in Gansu Higher Education Institutions [2018B-014]
Cynaropicrin exerts anti-proliferative and proapoptotic effects on H1975 and H460 lung cancer cells by deactivating the EGFR/AKT signaling pathway. It also upregulates the expressions of miR-202 and miR-370 in these cells, indicating its potential for lung cancer treatment.
Purpose: To investigate the anti-proliferative effect of cynaropicrin on lung cancer cell lines, and the underlying molecular mechanism. Methods: The effect of cynaropicrin treatment on the viabilities of H1975 and H460 cells was measured using Cell Counting Kit- 8. Apoptosis was analysed by annexin-V/FITC staining, while protein expressions were assayed by western blotting. Results: Treatment of H1975 and H460 cells with cynaropicrin at doses of 0.25 - 2.0 mu M led to a marked reduction in their viability (p < 0.05). In cynaropicrin-treated H1975 and H460 cells, there was significant increase in apoptosis, when compared to control cells. Caspase-3 and caspase-9 levels were also significantly increased in H1975 and H460 cells on treatment with cynaropicrin at doses of 0.25 and 2.0 mu M while treatment with cynaropicrin at doses of 0.25 - 2.0 mu M significantly down-regulated the mRNA expression of CCND1 in the two cell lines (p < 0.05). Cynaropicrin markedly inhibited mRNA and protein expressions of EGFR, and also downregulated AKT in H1975 and H460 cells (p < 0.05). However, cynaropicrin significantly increased the expressions of miR-202 and miR-370. Conclusion: Cynaropicrin exerts anti-proliferative and proapoptotic effects on H1975 and H460 lung cancer cells via deactivation of EGFR/AKT signaling pathway. Moreover, it upregulated the expressions of miR-202 and miR-370 in these cells. Thus, cynaropicrin has potentials for the treatment of lung cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据