期刊
TRENDS IN CELL BIOLOGY
卷 32, 期 5, 页码 445-461出版社
CELL PRESS
DOI: 10.1016/j.tcb.2022.01.009
关键词
endogenous PI metabolism; physiological context
类别
资金
- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program [101001097]
- ANR caLIPSO [ANR-18-CE13-0025-02]
- ANR STAYING-TIGHT [ANR-18-CE13-0016-02]
- ANRJC/JC Junior Investigator Grant [ANR-16-CE13-0021]
- SEED Fund ENS Lyon-2016
- SEED Fund ENS Lyon-2020
- ANR PlantScape [ANR-20-CE13-0026-02]
- ANR DIVCON [ANR-21-CE13-0016-03]
- French Ministry of Research and Higher Education
- European Research Council (ERC) [101001097] Funding Source: European Research Council (ERC)
- Agence Nationale de la Recherche (ANR) [ANR-20-CE13-0026, ANR-16-CE13-0021] Funding Source: Agence Nationale de la Recherche (ANR)
Phosphoinositides play critical roles in various biological processes and manipulating their levels accurately is crucial for understanding their functions. This review discusses different methods to manipulate phosphoinositide levels and highlights recent approaches in multicellular organisms.
Phosphoinositides (PIs) have critical roles in various cellular, physiological, developmental, pathological, and infectious processes. They are signaling phospholipids that can affect every aspect of membrane biology, including protein function (e.g., recruitment and activity), membrane physicochemical properties (e.g., curvature, surface charges, and packing), and the generation of secondary messengers. PIs act at precise locations within the cell in a dose-dependent manner, and their local concentration can vary drastically during signaling and trafficking. Thus, techniques able to manipulate PI amounts acutely and with subcellular accuracy are paramount to understanding the role of these lipids in vivo. Here, we review these methods and emphasize approaches recently developed to perturb PI levels in multicellular organisms.
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