期刊
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
卷 108, 期 -, 页码 214-221出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2014.12.016
关键词
Polycyclic aromatic hydrocarbon; Naphthenic acids; Oil sands process waters; Anaerobic biodegradation
资金
- NERC [NE/H017542/1]
- Oil Plus Ltd.
- University of Essex
- NERC [NE/M005712/1, NBAF010002, NE/I001352/1, NE/K000497/1] Funding Source: UKRI
- Natural Environment Research Council [NE/M005712/1, NE/K000497/1, NBAF010002, NE/I001352/1] Funding Source: researchfish
Both polycyclic aromatic hydrocarbons (PAHs) and naphthenic acids (NAs) are natural components of fossil fuels, but they are also widespread toxic and environmentally persistent pollutants. They are the major cause of environmental toxicity in oil-sands-process waters (OSPW). This study aimed to investigate the anaerobic biodegradation of the PAHs pyrene and 2-methylnaphthalene, and the NAs adamantane-1-carboxylic acid and a natural NA mixture (i.e., acid-extractable NAs from OSPW) under sulfate-reducing and methanogenic conditions by a microbial community derived from an oil sands tailings pond. Using gas-chromatography mass spectrometry (GC MS), the rate of biodegradation was measured in relation to changes in bacterial community composition. Only 2-methylnaphthalene was significantly degraded after 260 days, with significantly more degradation under sulfate-reducing (40%) than methanogenic conditions (25%). During 2-methylnaphthalene biodegradation, a major metabolite was produced and tentatively identified as 2-naphthoic acid. Denaturing gradient gel electrophoresis (DGGE) demonstrated an increase in intensity of bands during the anaerobic biodegradation of 2-methylnaphalene, which derived from species of the genera Fusibacter, Alkaliphilus, Desulfobacterium, Variovorax, Thaurea, and Hydrogenophaga. Despite the biodegradation of 2-methylnaphthalene, this study demonstrates that, under anaerobic conditions, NAs and high-molecular-weight PAHs are the predominant molecules likely to persist in OSPW. Therefore alternative remediation strategies are required. (C) 2015 Published by Elsevier Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据