期刊
TRANSBOUNDARY AND EMERGING DISEASES
卷 69, 期 5, 页码 2634-2648出版社
WILEY-HINDAWI
DOI: 10.1111/tbed.14416
关键词
chicken ISG12(2); immunity; Newcastle disease virus; vaccine; virulence
资金
- National Natural Science Foundation of China [31572538, 32002280]
- Natural Science Basic ResearchProgramof Shaanxi [2019JQ095]
- ChinaPostdoctoral Science Foundation [2019M660268]
This research identified chicken interferon-stimulated gene 12-2 as a potential adjuvant that can attenuate the virulence of Newcastle disease virus and increase the immunogenicity of the vaccine strain. It may contribute to the development of efficient poultry vaccines.
Low virulence and strong immunogenicity are quite important for Newcastle disease virus (NDV) producing Newcastle disease (ND) living-attenuated vaccine. However, immunogenicity of NDV positively correlates to its virulence. Usually, the velogenic NDV induces stronger immune responses of poultry than the lentogenic strain, but virulent NDV poses a risk for chicken. In this study, we identified the chicken interferon (IFN)-stimulated gene 12-2 (ISG12(2)) not only attenuated NDV, but also increased immunogenicity of ND vaccine strain. First, we found that NDV infection or IFNs stimulation induced expression of chicken ISG12(2) that reinforced expression of IFNs. Over-expression or knock-down proved that chicken ISG12(2) inhibited NDV replication. Then, recombinant NDV LaSota strains (rLaSota/Fmut/ISG12(2) and rLaSota/ISG12(2)), expressing ISG12(2), were rescued. Pathogenicity tests showed that ISG12(2) expression attenuated NDV. RNA-seq or RT-qPCR demonstrated that, comparing to rLaSota/Fmut and rLaSota, rLaSota/Fmut/ISG12(2) and rLaSota/ISG12(2) induced hosts to produce cytokines enriching in innate and adaptive immune pathways in vitro and in vivo. Finally, we showed that rLaSota/ISG12(2) vaccination improved immune condition of chicken to quickly respond NDV infection and then enhance protection. These results suggest that chicken ISG12(2) is a potential novel molecular adjuvant to regulate immune responses, which decrease virulence and increase immunogenicity of NDV. The chicken ISG12(2) may contribute to the development of high efficient poultry vaccine.
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