期刊
TOXICON
卷 205, 期 -, 页码 71-78出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2021.11.121
关键词
Scorpion venom; Hottentotta rugiscutis; MALDI-TOF MS; SDS-PAGE; Potassium channel toxins; Immunogenicity
资金
- SERB-DST, New Delhi [EEQ/2017/000049]
This study delves into the venom composition and immunogenicity of the Hottentotta rugiscutis scorpion, revealing diverse protein components and potential seasonal variations in the presence of K+ or Na+ channel blockers. The venom also induces a stress-induced innate immune response in mice, leading to a strong humoral immune response.
The scorpions of the Buthidae family exhibit diverse toxins with proven pharmacological activities and yet underexplored. The Hottentotta rugiscutis is a commonly found south-Indian buthid scorpion, whose venom proteomic profile is unknown. In this study, the venom was biochemically and immunologically characterized by SDS-PAGE, MALDI-TOF MS, Western blot and ELISA. The regional and seasonal variation in the venom composition from the same species was also assessed at the molecular mass level. The venom was further studied in albino mice to understand its impact on various blood parameters. The venom has varied MW proteins from 6 to 275 kDa, four of them were found to be major immunodominant proteins. The mass spectra have revealed that some proteins are predominantly present in the venom of 3-4.5 kDa or 6.5-8.0 kDa, which could be the K+ or Na+ channel blockers respectively whose ratio varied by season. The obtained venom-mass spectra could also be used as H. rugiscutis specific finger-print in identifying the region-specific species. The venom was found to elicit a stress-induced innate immune response in mice, giving rise to a strong Th2 mediated humoral immune response. Overall, this study has provided a glimpse of the venom composition and its immunogenicity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据