期刊
TOXICOLOGY
卷 464, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2021.153013
关键词
Patulin; Oocyte quality; Meiotic progression; Oxidative stress; Mitochondria
Patulin treatment inhibits mouse oocyte maturation and causes meiotic arrest by disrupting normal spindle assembly and chromosome alignment, which may be related to mitochondrial dysfunction. Increased oxidative stress and decreased mitochondrial membrane potential are also observed in patulin-treated oocytes.
Patulin is a secondary metabolite mainly secreted by fungi and is the most common mycotoxin found in apples and apple-based products. For the past few years, numerous studies suggested the wide distribution and toxicity of patulin. In this study, we investigated the toxic effect of patulin on mouse oocytes and its possible mechanisms. The results showed that patulin treatment did not affect meiotic resumption, but inhibited oocyte maturation as indicated by failure of first polar body extrusion. Further mechanistic study showed that patulin treatment disturbed normal spindle assembly, chromosome alignment and morphology. We also found increased oxidative stress by testing the level of ROS and decreased mitochondrial membrane potential, indicating mitochondria dysfunction. In summary, our results suggest that patulin treatment causes oocyte meiotic arrest by disturbing normal spindle assembly and chromosome alignment, which may be caused by dysfunctions of mitochondria.
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