期刊
TOXICOLOGY
卷 464, 期 -, 页码 -出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2021.153019
关键词
Cassava; CCE; Cyanogenic glycosides; A549; Cytotoxicity
资金
- University Grants Commission, New Delhi
- Biomedical Technology Wing, Sree ChitraTirunal Institute for Medical Sciences and Technology (Govt. of India)
- ICAR-CTCRI, Trivandrum, Kerala, India
Cassava, an important root crop in tropical countries, was formulated into biopesticide CCE to combat borer insect pests. Cell studies showed dose-dependent cytotoxicity of CCE on A549 cells, with different effects on free radical scavenging and nitrite radicals, and a negative correlation with mitochondrial and lysosomal functions. Higher concentrations of CCE led to necrotic cell death rather than apoptosis, indicating significant anti-proliferative ability.
Cassava (Manihotesculenta Crantz) is one of the most important root crops in tropical countries. It is a major source of cyanogenic glycosides viz. linamarin and lotaustralin, and these on breakdown liberate HCN and ketone. Cassava cyanide extract (CCE) from cassava leaves and tuber rinds were formulated as a biopesticide against certain borer insect pests of horticultural crops. Adenocarcinomic human alveolar basal epithelial cells (A549) were treated with three different concentrations (100, 200, 400 ppm) of CCE. The MTT and NRU assays showed dose-dependent cytotoxicity. The DCFH-DA assay does not show any free radical scavenging activity, whereas the NRR assay showed a reduction in the nitrite radicals with an increase in the concentration of the bioactive compound. A negative correlation was found between the concentration of the bioactive principles and mitochondrial and lysosomal functions. Various cellular assays demonstrated the cellular response of the CCE, and it was found that at higher concentration (400 ppm), the CCE exert a significant necrotic cell death rather than apoptosis. The results of the study indicated that the CCE have a remarkable tendency of anti-proliferative ability.
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