期刊
TOXICOLOGICAL SCIENCES
卷 185, 期 1, 页码 77-88出版社
OXFORD UNIV PRESS
DOI: 10.1093/toxsci/kfab121
关键词
QuEChERS; nontargeted analysis; GC-Orbitrap; exposome; persistent organic pollutants
类别
资金
- National Institute of Environmental Health Sciences (NIEHS) Training in Environmental Pathology T32 program [T32 ES007272]
- NIEHS [R01 ES024946]
- USDA National Institute of Food and Agriculture, Hatch Project [1002182]
- NIFA [689961, 1002182] Funding Source: Federal RePORTER
This study presents a high-resolution mass spectrometry method for both targeted and nontargeted analysis of trace organic contaminants using QuEChERS extraction. The method performs well in the analysis of both target and nontarget compounds in serum samples. The study identified a large number of chemicals in human exposome and categorized them accordingly.
Humans are exposed to a broad range of organic chemicals. Although targeted gas chromatography mass spectrometry techniques are used to quantify a limited number of persistent organic pollutants and trace organic contaminants in biological samples, nontargeted, high-resolution mass spectrometry (HRMS) methods assess the human exposome more extensively. We present a QuEChERS extraction for targeted and nontargeted analysis of trace organic contaminants using HRMS and compare this method to a traditional, cartridge-based solid-phase extraction (SPE). Following validation using reference and spiked serum samples, the method was applied to plasma samples (n = 75) from the Prospective investigation of Obesity, Energy, and Metabolism (POEM) study. We quantified 44 analytes using targeted analysis and 6247 peaks were detected using the nontargeted approach. Over 90% of targeted analytes were at least 90% recovered using the QuEChERS method in spiked serum samples. In nontargeted analysis, 84% of the peaks were above the method detection limit with area counts up to 3.0 x 10(5) times greater using the QuEChERS method. Of the targeted compounds, 88% were also identified in the nontargeted analysis. We categorized the 4212 chemicals assigned an identity in using EPA's CompTox Dashboard and 1076 chemicals were found in at least one list. The category with the highest number of chemicals was androgen or estrogen receptor activity. The findings demonstrate that a QuEChERS technique is suitable for both targeted and nontargeted analysis of trace organic contaminants in biological samples.
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