期刊
THERIOGENOLOGY
卷 173, 期 -, 页码 211-220出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2021.06.025
关键词
ACTH; Apoptosis; CRH; Oocyte competence; Stress
资金
- China National Natural Science Foundation of China [31702114, 31902160, 31772599, 32072738]
- National Key R&D Program of China [2017YFC1001601, 2017YFC1001602]
- Natural Science Foundation of Shandong Province [ZR2017BCE025]
- Funds of Shandong Double Tops Program [SYL2017YSTD12]
Research shows that CRH impairs the competence of pig and mouse oocytes and induces apoptosis. While mouse cumulus cells undergo apoptosis, pig cumulus cells do not.
Although it is known that stresses on females damage oocytes with increased production of stress hormones, whether corticotrophin-releasing hormone (CRH) or adrenocorticotropic hormone (ACTH) harm oocytes directly are largely unknown. We demonstrated that CRH exposure during in vitro maturation impaired competence of both pig and mouse cumulus-oocyte-complexes (COCs), and it impaired competence and induced apoptosis in pig cumulus-denuded oocytes (DOs) but not in mouse DOs. CRH receptor 1 was expressed in pig DOs and in cumulus cells (CCs) of both species but not in mouse DOs. In the presence of CRH, whereas mouse CCs underwent apoptosis, pig CCs did not. While pig CCs did, mouse CCs did not express CRH-binding protein. ACTH did not affect competence of either pig or mouse COCs or DOs although they all expressed ACTH receptor. Both pig and mouse CCs expressed steroidogenic acute regulatory protein (StAR), and ACTH enhanced their progesterone production while alleviating their apoptosis. Neither pig nor mouse DOs expressed StAR, but ACTH inhibited maturation promoting factor and decelerated meiotic progression of DOs suggesting activation of protein kinase A (PKA). In conclusion, CRH impaired pig and mouse oocyte competence by interacting with CRH receptor and inducing CCs apoptosis, respectively. ACTH activated PKA in both DOs and CCs although it showed no effect on oocyte competence. (c) 2021 Elsevier Inc. All rights reserved.
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