Development of a miniature protein mass spectrometer capable of analyzing native proteins

Current miniature mass spectrometers were usually designed for the detection of small and medium size molecules, including volatile (semi-volatile) compounds, drugs and lipids. In this study, a miniature protein mass spectrometer was developed in this work, which could serve as a biosensor for the rapid identification of proteins as well as their conformations. A linear ion trap with a field radius of 2.5 mm was designed to extend mass range of the instrument to over 6500 Th. Mass resolution and sensitivity of the instrument were also optimized for protein ions by increasing the buffer gas pressure and using a high-gain Faraday detector. It is then demonstrated that the mass spectra of native proteins, such as IgG1, could be acquired by coupling the instrument with a soft electrospray ionization source. As a proof-of-concept demonstration, results suggest that the current instrument could be used to identify target proteins and probe/distinguish their conformations in solutions.

Automated summary

A miniature protein mass spectrometer was developed for rapid identification of proteins and their conformations, with high mass resolution and sensitivity. Coupling the instrument with a soft electrospray ionization source allowed the acquisition of mass spectra of native proteins.