☆ 4.7 Article

A strategy combining 3D-DNA Walker and CRISPR-Cas12a trans-cleavage activity applied to MXene based electrochemiluminescent sensor for SARS-CoV-2 RdRp gene detection

TALANTA (2022)

相关参考文献

注意：仅列出部分参考文献，下载原文获取全部文献信息。

Article Biophysics

Exploring the trans-cleavage activity of CRISPR-Cas12a for the development of a Mxene based electrochemiluminescence biosensor for the detection of Siglec-5

Kai Zhang et al.

Summary: Siglec-5 is an inhibitory receptor for leukocytes in the human body, and the development of novel assays for Siglec-5 can aid in studying related disease pathogenesis and developing therapeutic drugs. A biosensor based on CRISPR-Cas12a and MXene has been developed for sensitive detection of Siglec-5, extending the application of CRISPR-Cas12a to biosensor construction and providing a common method for biomarker detection. This work represents the first investigation into the Trans-Cleavage activity of CRISPR-Cas12a for Mxene-based ECL biosensor establishment.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Chemistry, Analytical

Ultrasensitive supersandwich-type electrochemical sensor for SARS-CoV-2 from the infected COVID-19 patients using a smartphone

Hui Zhao et al.

Summary: The study developed an ultrasensitive electrochemical detection technology using calixarene functionalized graphene oxide for targeting SARS-CoV-2 RNA without the need for nucleic acid amplification and reverse transcription. The biosensor demonstrated high specificity and selectivity, offering a rapid and convenient method for SARS-CoV-2 detection at the point of care.

SENSORS AND ACTUATORS B-CHEMICAL (2021)

添加到收藏夹

Article Biophysics

Entropy-driven electrochemiluminescence ultra-sensitive detection strategy of NF-κB p50 as the regulator of cytokine storm

Kai Zhang et al.

Summary: In this study, an entropy-driven electrochemiluminescence (ECL) biosensor was constructed for ultra sensitive bioassay of NF-kappa B p50, providing a repeatable approach for TFs detection.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Biophysics

A signal-on photoelectrochemical aptasensor for chloramphenicol assay based on 3D self-supporting AgI/Ag/BiOI Z-scheme heterojunction arrays

Jian-Hong Zhu et al.

Summary: A novel signal-on PEC aptasensor was developed for highly sensitive and selective detection of chloramphenicol. The sensor achieved a wide linear range of 2-250 nM and a low limit of detection of 0.226 nM through the combination of 3D self-supporting AgI/Ag/BiOI Z-scheme heterojunction arrays and BCP signal amplification strategy. This work opens up new possibilities for PEC aptasensing technology and demonstrates potential for bioanalysis of environmental samples.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Preanalytical Issues and Cycle Threshold Values in SARS-CoV-2 Real-Time RT-PCR Testing: Should Test Results Include These?

Ilka Engelmann et al.

Summary: Since the outbreak of the SARS-CoV-2 pandemic, clinical laboratories have been overwhelmed with testing, leading to shortages in reagents. Different viral inactivation procedures and transport buffers can help alleviate the bottleneck for PCR-based methods. However, finding efficient alternative lysis buffers remains challenging, especially in the current crisis.

ACS OMEGA (2021)

添加到收藏夹

Review Biophysics

CRISPR-based detection of SARS-CoV-2: A review from sample to result

Reza Nouri et al.

Summary: This work reviews and summarizes the applications of CRISPR technology in the context of the COVID-19 pandemic, highlighting its potential for rapid and accurate detection of SARS-CoV-2, as well as providing future prospects for point-of-care molecular diagnosis applications.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Biophysics

Entropy-driven amplified electrochemiluminescence biosensor for RdRp gene of SARS-CoV-2 detection with self-assembled DNA tetrahedron scaffolds

Zhenqiang Fan et al.

Summary: A novel sensor for SARS-CoV-2 detection has been established utilizing an entropy-driven amplified electrochemiluminescence strategy, based on DNA tetrahedron design. By avoiding expensive enzyme reagents, the sensor efficiently detects viral RNA with high specificity and sensitivity, providing a reliable platform for large-scale screening of COVID-19 patients.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Chemistry, Physical

CRISPR-Cas12a-driven MXene-PEDOT:PSS piezoresistive wireless biosensor

Ruijin Zeng et al.

Summary: This study introduces a CRISPR-Cas12a-based instrument-free point-of-care detection technology, which uses smartphone for visualized DNA detection results, and combines wireless sensing technology, significantly improving portability and detection sensitivity.

NANO ENERGY (2021)

添加到收藏夹

Review Chemistry, Multidisciplinary

CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules

Wei Feng et al.

Summary: The advancement of CRISPR-Cas technology has revolutionized genome editing and transcriptional modulation, as well as molecular detection of diverse targets. Integrating CRISPR-Cas systems with nucleic acid amplification strategies enables amplified detection signals, enrichment of low-abundance targets, improved specificity and sensitivity, and development of point-of-care diagnostic techniques.

CHEMICAL SCIENCE (2021)

添加到收藏夹

Article Nanoscience & Nanotechnology

Dual-Wavelength Electrochemiluminescence Ratiometric Biosensor for NF-κB p50 Detection with Dimethylthiodiaminoterephthalate Fluorophore and Self-Assembled DNA Tetrahedron Nanostructures Probe

Zhenqiang Fan et al.

ACS APPLIED MATERIALS & INTERFACES (2020)

添加到收藏夹

Article Medical Laboratory Technology

Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia

Daniel K. W. Chu et al.

CLINICAL CHEMISTRY (2020)

添加到收藏夹

Article Infectious Diseases

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Victor M. Corman et al.

EUROSURVEILLANCE (2020)

添加到收藏夹

Article Chemistry, Multidisciplinary

Dual-Functional Plasmonic Photothermal Biosensors for Highly Accurate Severe Acute Respiratory Syndrome Coronavirus 2 Detection

Guangyu Qiu et al.

ACS NANO (2020)

添加到收藏夹

Article Chemistry, Multidisciplinary

Selective Naked-Eye Detection of SARS-CoV-2 Mediated by N Gene Targeted Antisense Oligonucleotide Capped Plasmonic Nanoparticles

Parikshit Moitra et al.

ACS NANO (2020)

添加到收藏夹

Article Chemistry, Multidisciplinary

CRISPR/Cas13a Powered Portable Electrochemiluminescence Chip for Ultrasensitive and Specific MiRNA Detection

Ting Zhou et al.

ADVANCED SCIENCE (2020)

添加到收藏夹

Review Biophysics

Detection of COVID-19: A review of the current literature and future perspectives

Tianxing Ji et al.

BIOSENSORS & BIOELECTRONICS (2020)

添加到收藏夹

Article Biophysics

Homogeneous circle-to-circle amplification for real-time optomagnetic detection of SARS-CoV-2 RdRp coding sequence

Bo Tian et al.

BIOSENSORS & BIOELECTRONICS (2020)

添加到收藏夹

Article Virology

A reverse-transcription recombinase-aided amplification assay for the rapid detection of N gene of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)

Tao Wu et al.

VIROLOGY (2020)

添加到收藏夹

Article Biophysics

Ultrasensitive detection of hERG potassium channel in single-cell with photocleavable and entropy-driven reactions by using an electrochemical biosensor

Kai Zhang et al.

BIOSENSORS & BIOELECTRONICS (2019)

添加到收藏夹

Review Chemistry, Analytical

Research progress of DNA walker and its recent applications in biosensor

Jing Chen et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2019)

添加到收藏夹

Article Chemistry, Analytical

Perylene Derivative/Luminol Nanocomposite as a Strong Electrochemiluminescence Emitter for Construction of an Ultrasensitive MicroRNA Biosensor

Wei Liu et al.

ANALYTICAL CHEMISTRY (2019)

添加到收藏夹

Article Chemistry, Analytical

Electrochemiluminescence Peptide-Based Biosensor with Hetero-Nanostructures as Coreaction Accelerator for the Ultrasensitive Determination of Tryptase

Fang-Fang Wu et al.

ANALYTICAL CHEMISTRY (2018)

添加到收藏夹

Review Chemistry, Analytical

Emerging bioanalytical applications of DNA walkers

Sean D. Mason et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2018)

添加到收藏夹

Article Chemistry, Multidisciplinary

Rational Engineering of a Dynamic, Entropy-Driven DNA Nanomachine for Intracellular MicroRNA Imaging

Cheng-Pin Liang et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

添加到收藏夹

Article Chemistry, Multidisciplinary

Ultrasensitive fluorescence detection of transcription factors based on kisscomplex formation and the T7 RNA polymerase amplification method

Kai Zhang et al.

CHEMICAL COMMUNICATIONS (2017)

添加到收藏夹

Article Chemistry, Multidisciplinary

A catalytic assembled enzyme-free three-dimensional DNA walker and its sensing application

Wei Li et al.

CHEMICAL COMMUNICATIONS (2017)

添加到收藏夹

Article Nanoscience & Nanotechnology

A stochastic DNA walker that traverses a microparticle surface

C. Jung et al.

NATURE NANOTECHNOLOGY (2016)

添加到收藏夹

Article Chemistry, Multidisciplinary

Enzyme-Powered Three-Dimensional DNA Nanomachine for DNA Walking, Payload Release, and Biosensing

Xiaolong Yang et al.

ACS NANO (2016)

添加到收藏夹

Article Chemistry, Analytical

Electrogenerated Chemiluminescence Peptide-Based Biosensor for the Determination of Prostate-Specific Antigen Based on Target-Induced Cleavage of Peptide

Honglan Qi et al.

ANALYTICAL CHEMISTRY (2014)

添加到收藏夹

© Peeref 2019-2024. All rights reserved.