期刊
SYNAPSE
卷 76, 期 5-6, 页码 -出版社
WILEY
DOI: 10.1002/syn.22227
关键词
acid-sensing ion channel (ASIC); drug action; glutamate; ligand-gated ion channel; pharmacology
资金
- Russian Science Foundation [21-14-00280]
- Russian Science Foundation [21-14-00280] Funding Source: Russian Science Foundation
Glutamate potentiates the activity of ASIC1a, allowing it to function in glutamatergic synapses. This potentiation occurs through a shift in the pH-dependent activation threshold.
Acid-sensing ion channels (ASICs) participate in synaptic transmission due to the acidic content of synaptic vesicles, but their contribution to postsynaptic currents is small. This has stimulated attempts to find endogenous ASIC potentiators that could enhance ASIC-mediated currents to physiologically relevant values. Here we demonstrate that glutamate, which serves as a neurotransmitter, potentiates recombinant ASIC1a in the submillimolar concentration range. The effect of glutamate is especially interesting as ASIC's presence has been shown in glutamatergic synapses. At pH=6.5 glutamate had maximum potentiation of 87% with an EC50 value of 0.65 mM. The mechanism of potentiation is due to a shift of pH-dependent activation to less acidic values, with 0.5 mM glutamate increasing pH(50) from 6.04 to 6.43. Due to this mechanism, ASIC1a in glutamatergic synapses might be intrinsically potentiated. Furthermore, this effect could compensate for the inhibition of ionotropic glutamate receptors by extracellular acidification during synaptic transmission.
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