期刊
STEM CELL RESEARCH
卷 56, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.scr.2021.102511
关键词
alpha-glucosyl-rutin; Induced pluripotent stem cells; Keratinocytes; Immediate early genes
资金
- Kato Memorial Bioscience Foundation
- Japan Atomic Energy Agency
- Japan Society for the Promotion of Science [JP15H02817, JP17K20042, JP20H04334]
Research indicates that AGR can increase the viability of induced pluripotent stem cells (iPSCs) and induce the expression of genes related to differentiation.
Rutin is a natural flavonoid glycoside found in several vegetables and fruits such as buckwheat and onion. Rutin has a range of pharmacological effects that include anti-oxidant, anti-inflammation, anti-bacterial, and anti-cancer activities. alpha-glucosyl-rutin (AGR) is a derivative of rutin with increased water solubility that is used in cosmetics and foods. However, the effects of AGR on cellular responses have not been clarified, especially in stem cells. Induced pluripotent stem cells (iPSCs) show high proliferative activity and pluripotency; however, regulation of molecular machinery such as cell cycle, metabolism, and DNA repair differs between iPSCs and somatic cells. Here, we compared the effects of AGR on iPSCs and differentiated cells (fibroblasts and skin keratinocytes). AGR-treated iPSCs exhibited increased cell viability. RNA sequencing and reverse transcriptase PCR analysis revealed that AGR induced expression of immediate early genes (IEGs) and differentiation-related genes in iPSCs. Our results suggest that AGR may activate differentiation signals mediated by IEG responses in iPSCs, resulting in altered metabolic activity and increased cell viability.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据