期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 260, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2021.119988
关键词
1; 3; 5-Triazine; Fluorescent probe; Stokes shift; Lipid droplet; Cell imaging
类别
资金
- Natural Science Foundation of China [21801145]
- Shandong Provincial Key R&D Program/Major Science and Technology Innovation Project of Shandong Province [2019JZZY020230]
- Natural Science Foundation of Shandong Province [ZR2017BB012]
- Program for Science and Technology Innovation Talents in Universities of Henan Province [20HASTIT037]
LDs play a crucial role in protein transformation and maturation in living bodies, with imbalances potentially leading to serious diseases. The diameter distribution of LDs ranges from 100 nm to 100 μm, and the novel organic bio-probe TzAr-N exhibits favorable LDs targeting ability and low biological toxicity.
Lipid droplets (LDs) including triacylglycerols and cholesteryl esters are applied for protein transformation and protein maturation in living bodies, which have an important effect on lipid metabolism, homeostasis, and interactions with other organelles. The imbalance of LDs could lead to many serious diseases including insulin resistance, cancer, obesity, liver disease, cardiovascular disease, and neurodegenerative disease. The diameter distribution of LDs is quite extensive from 100 nm to 100 mu m. Herein, we designed and constructed a novel organic bio-probe TzAr-N for LDs cell imaging with much more hydrophobic and viscous environment compared to cytosol by using 1,3,5-triazine as electron acceptor. This sensitive probe exhibited favorable merits including large Stokes shift (almost 80 nm), good LDs targeting ability, and a low biological toxicity, which also could not be affected by wide range of pH values. Furthermore, the bio-probe TzAr-N could also mark LDs distribution in living HeLa cells. (c) 2021 Elsevier B.V. All rights reserved.
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