Effect of caffeine on the possible amelioration of diabetic neuropathy: A spectroscopic study

Importance: One of the consequential and alarming complications of diabetes mellitus is diabetic neuropathy (DN). DN is assured to be caused chiefly by excess sorbitol levels in the body. The harmful consequences of DN alike peripheral nerve damage with extremity ulcers may be dodged with timely detection and treatment. The therapeutic methods for DN are scarce and expensive. Therefore economic and user friendly methodologies to prevent acquiring the disease need proper attention. Objective: The present research has been conducted (1) to analyse the levels of sorbitol in diabetic blood samples and compare them with non-diabetic ones and (2) to study the reduction in sorbitol levels upon addition of an important biochemical compounds caffeine in both sample groups. Research design, setting, participants and method: Sorbitol-caffeine interaction analysis of blood samples of 16 patients with type 2 diabetes from KPC Medical College, Kolkata, India was made. The spectroscopic analysis and their interpretations were compared with 16 healthy subjects. Main outcomes and measures: Present work describes that caffeine can be helpful in reducing the sorbitol level in diabetics, so the chances of development and progression of diabetic neuropathy can be controlled with the introduction of caffeine. Results: A total number of 32 blood samples of patients (aged 35-70 years); mean age ranges were 52.06 +/- 2.68 and 53.50 +/- 2.66 years for non-diabetic and diabetic ones respectively, glucose and sorbitol screening examination were done by enzymatic methodologies where concentrations were assessed by means of either absorption or fluorescence spectroscopy. The calibration range was 18.2-1119.3 mg/dL (Linear regression analysis r(2) = 0.996). The sensitivity of this screening program in detecting DN with the healthy adults has been inquired and found efficient. Results of fasting insulin analyses have also been analysed for HOMA-IR (homeostasis model assessment - insulin resistance) and HOMA-B (homeostasis model assessment - pancreatic beta cell function) values. Statistical significance of the results in non-diabetic and diabetic groups were performed and found to be statistically significant. Conclusions: We have defined the relationship between blood glucose level, insulin level, sorbitol and caffeine in human body and utilized them in the plausible remediation of DN. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

The study focused on analyzing sorbitol levels in diabetic blood samples and comparing them with non-diabetic samples, as well as studying the reduction in sorbitol levels upon the addition of caffeine. The results showed that caffeine could potentially help reduce sorbitol levels in diabetic patients, thus controlling the development and progression of diabetic neuropathy.