期刊
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
卷 261, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2021.120024
关键词
Fluorescent probe; Ratiometric; Hypochlorite; Lysosome; Targeting; Cell imaging
类别
资金
- National Natural Science Foundation of China [22008222]
A lysosomal-targeted fluorescent probe Cou-Lyso was developed for real-time detection of ClO- in cells, showing high sensitivity and low detection limit. Confocal imaging experiments demonstrated that this probe could detect ClO- in living cells and zebra fish, providing a promising tool for studying ClO- in complex biological systems.
Hypochlorite (ClO-), a type of reactive oxygen species (ROS), plays an essential role in complex biological systems. Real-time detection of the content and distribution of ClO- in cells or subcellular organelle is critically essential. In this paper, a lysosomal-targeted fluorescent probe, Cou-Lyso, was constructed for real-time detection of ClO- in a ratiometric manner, achieving high sensitivity with a low detection limit (0.58 mu M). Upon reaction with ClO-, this probe was subjected to a significant fluorescence change from red emission (lambda(max)em = 610 nm) to green emission (lambda(max)em = 535 nm) with the ratio of I-535 (nm)/I-610 nm displaying a 76-fold enhancement from 0.04 to 3.03. The confocal imaging experiments for Cou-Lyso showed that this probe could detect ClO- in living cell and zebra fish. This probe has been successfully applied to stain lysosome and image lysosomal ClO- based on co-localization imaging experiments. (C) 2021 Elsevier B.V. All rights reserved.
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