Fluorescence discrimination of HSA from BSA: A close look at the albumin-induced restricted intramolecular rotation of flavonoid probe

Discrimination of human serum albumin (HSA) from bovine serum albumin (BSA) based on the fluores-cence probe technique is still challenging due to similar chemical structures. In this work, a novel flavonoid-based fluorescent probe AF is reported for successful discrimination of HSA from BSA. The sens -ing performances of probe, including sensing dynamic, sensitivity and selectivity, have been carefully studied. Moreover, sensing mechanism was elucidated by Job's plot, displacement experiment, and molecular docking, suggesting that the specific response to HSA originated from the albumin-induced restricted intramolecular rotation (RIR) of probe. This work may provide a simple way for designing of novel probes for HSA with high selectivity. (c) 2021 Published by Elsevier B.V.

Automated summary

This study presents a novel fluorescent probe AF for discriminating HSA from BSA, and investigates the sensing performance and mechanism of the probe. The results elucidate that the specific response to HSA is due to the albumin-induced restricted intramolecular rotation (RIR) of the probe, providing insight for designing high-selectivity probes for HSA.