A fluorogenic H2S donor activated by reactive oxygen species for real-time monitoring in cells and in vivo

Hydrogen sulfide (H2S) is an important gasotransmitter in biological system, and plays a crucial role in varied physiological and pathological processes. Exogenous H2S is widely employed as a positive control in H2S related biological study. Herein, we develop a reactive oxygen species (ROS) triggered donor HSD545 that delivers H2S and simultaneously generates a fluorophore to real-time monitoring the process of H2S release in vitro and in vivo. The donor exhibits low cytotoxicity and strong cytoprotection against ROS-induce oxidative stress. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

Hydrogen sulfide (H2S) is a crucial gasotransmitter in biological systems and is widely used in biological studies. A newly developed donor molecule, HSD545, triggers the release of H2S in response to reactive oxygen species (ROS) and simultaneously generates a fluorescent substance for real-time monitoring. The donor exhibits low cytotoxicity and provides strong cytoprotection against ROS-induced oxidative stress.