Synthesis and Bio-evaluation of Soluble Sodium Copper Chlorophyllin Complexes from the Leaves of Aloe vera

Chlorophyllin is a semi-synthetic mixture of sodium copper complexes synthesized from chlorophyll. In the present investigation the copper-chlorophyllin (Cu-chlorophyllin) and soluble sodium copper chlorophyllin (Na-Cu-chlorophyllin) complexes were synthesized from leaves of Aloe vera. The synthesized compounds were estimated for the chlorophyll content. Na-Cu-chlorophyllin complexes showed high content of total chlorophyll (4.862 mg/mg). The synthesized compounds were characterized for its morphology by SEM, EDAX, FTIR and XRD. The complexes showed the presence of phytochemicals like phenols (21.62 mg/mL) and phytosterols (444.66 mg/mL) associated with it. The Na-Cu-chlorophyllin complexes showed antimicrobial activity with moderate results against S aureus, B cereus and maximum zone of inhibition against S typhi (23.6 +/- 0.33 mm).The complexes showed excellent free radical scavenging activity for DPPH with 61.84 % scavenging and IC50 value of 33.74 mg/mL. The complexes also exhibited moderate lethality of 30 % on brine shrimp after 72 h with an LC50 value 328.94 mg/mL. Since, Na-Cuchlorophyllin complexes exhibited excellent bioactivity, it can be utilized extensively in various food, cosmetic, herbal medicine and pharma industries. (C) 2021 SAAB. Published by Elsevier B.V. All rights reserved.

Automated summary

In this study, copper-chlorophyllin and soluble sodium copper chlorophyllin complexes were synthesized from Aloe vera leaves and characterized. The synthesized complexes showed high chlorophyll content, antimicrobial activity, and excellent free radical scavenging activity. Therefore, they can be extensively utilized in the food, cosmetic, herbal medicine, and pharmaceutical industries.