期刊
SMALL
卷 18, 期 10, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202106281
关键词
biosensors; delivery vehicles; DNA-based nanoprobes; in situ detection; microRNA imaging
类别
资金
- Excellent Young Scientists Fund [22022407]
- National Natural Science Foundation of China [21874008, 22004006]
- Major Program of National Natural Science Foundation of China [21890740, 21890742]
- Special Foundation for State Major Research Program of China [2019YFC1606603]
- SZU Top Ranking Project [860000002100165, 86000000210]
- Beijing Municipal Science and Technology Commission [z131102002813058]
- 2021 Stability support plan of Shenzhen University [8940206/0200]
This review focuses on the application of DNA-based nanoprobes in miRNA biosensors, presenting strategies for customizing desired nanoplatforms including delivery vehicles, DNA sequence design, and advancements in imaging technologies for accurate signal output. Challenges and future directions in the field are also discussed.
DNA-based nanoprobes integrated with various imaging signals have been employed for fabricating versatile biosensor platforms for the study of intracellular biological process and biomarker detection. The nanoprobes developments also provide opportunities for endogenous microRNA (miRNA) in situ analysis. In this review, the authors are primarily interested in various DNA-based nanoprobes for miRNA biosensors and declare strategies to reveal how to customize the desired nanoplatforms. Initially, various delivery vehicles for nanoprobe architectures transmembrane transport are delineated, and their biosecurity and ability for resisting the complex cellular environment are evaluated. Then, the novel strategies for designing DNA sequences as target miRNA specific recognition and signal amplification modules for miRNA detection are presented. Afterward, recent advances in imaging technologies to accurately respond and produce significant signal output are summarized. Finally, the challenges and future directions in the field are discussed.
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