期刊
SMALL
卷 17, 期 52, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202102392
关键词
2D materials; cucurbituril; in situ generation; intracellular signaling; spiropyran
类别
资金
- National Natural Science Foundation of China [22078210]
- Sichuan Science and Technology Program [2020YJ0315]
- Engineering Innovation Team Project of Sichuan University [2020SCUNG122]
- Opening Project of Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University [SCU2021D005]
- Special Program for Technical Support of the State Administration for Market Regulation [2020YJ024]
By engineering cucurbit[6]uril with spiropyran pendants, large 2D materials are synthesized inside living cells, allowing for potent intracellular signaling or theranostic platform that exceeds the performance of conventional small counterparts.
The unique structural advantage and physicochemical properties render some 2D materials emerging platforms for intracellular bioimaging, biosensing, or disease theranostics. Despite recent advances in this field, one major challenge lies in bypassing the endocytic uptake barrier to allow internalization of very large 2D materials that have longer retention time in cells, and hence greater potency as intracellular functional platforms than small, endocytosable counterparts. Here, an engineered cucurbit[6]uril carrying at its periphery multiple spiropyran pendants that readily translocates into cytosol, and then polymerizes laterally and non-covalently in a controlled manner, enabling direct generation of 2D materials inside living cells, is reported. The resultant 2D materials are single-monomer-thick and can in situ grow up to 0.8-1.2 mu m in lateral size, experimentally proved too large to be endocytosed from outside the cells even after surface engineered with biorecognition entities. A Forster resonance energy transfer assay is further devised for real-time visualization of the polymerization dynamics in vivo, clearly demonstrating the rationale in this study. With the otherwise non-endocytosable large 2D materials gaining access to cytosol, potent intracellular signaling or theranostic platform that surpasses the intrinsic performance limit of conventional small counterparts are in sight.
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