期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 359, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2022.131596
关键词
SPR; Biosensor; Lung cancer gene detection; Optical fiber; Fiber Bragg grating
资金
- National Natural Science Foundation of China [61903073]
- China Postdoctoral Science Foundation
- Natural Science Foundation of Hebei Province [F2020501040]
- Fundamental Research Funds for the Central Universities [N2004011]
This paper demonstrates a novel DNA hybridization optical fiber sensor with temperature and pH compensation for detecting a common lung cancer gene. The sensor achieves a three-parameter detection of DNA sequence, temperature, and pH using a single fiber, and exhibits advantages of fast response, high sensitivity, low detection limit, and label-free detection.
A novel DNA hybridization optical fiber sensor with temperature and pH compensation for detecting a common lung cancer gene of epidermal growth factor receptor (EGFR) gene was demonstrated in this paper. The paper proposes the first research to achieve dual surface plasmon resonance (SPR) in an Fiber Bragg grating (FBG) and implement a three-parameter detection of DNA sequence, temperature and pH using a single fiber. Probe DNA sequences (pDNA) and PAA/CS, a pH-sensitive material, are immobilized on the sensor surface for EGFR gene exon-20 detection and temperature and pH monitoring simultaneously. The effectiveness of this functionalized method has been proved by using an atomic force microscope (AFM) and scanning electron microscope (SEM). The limit of detection (LOD) of EGFR gene was experimentally verified to 13.5 nM. The advantages of fast response, high sensitivity, label free, plug in, low LOD and three-parameter detection demonstrated by this sensor in the experiment are important for the field of DNA hybridization research and solving the problems of biosensor susceptibility to temperature and pH.
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