期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 352, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.131060
关键词
MERS-NV; SELEX; Surface-enhanced Raman spectroscopy; Aptamer; MERS-NV biosensor
资金
- Korea Environment Industry&Technology Institute (KEITI) through the program for the management of aquatic ecosystem health - Korea Ministry of Environment (MOE) [2020003030001]
- Industrial Core Technology Development Program - Ministry of Trade, Industry and Energy (MOTIE, Korea) [20009121]
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [2019R1A2C3002300]
- Korea Evaluation Institute of Industrial Technology (KEIT) [20009121] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A MERS-nanovesicle biosensor with multifunctional DNA aptamer was developed for detecting MERS-NVs in different solutions, offering a new approach for MERS-CoV detection.
Middle East respiratory syndrome coronavirus (MERS-CoV) is one of the most harmful viruses for humans in nowadays. To prevent the spread of MERS-CoV, a valid detection method is highly needed. For the first time, a MERS-nanovesicle (NV) biosensor composed of multi-functional DNA aptamer and graphene oxide encapsulated molybdenum disulfide (GO-MoS2) hybrid nanocomposite was fabricated based on electrochemical (EC) and surface-enhanced Raman spectroscopy (SERS) techniques. The MERS-NV aptamer was designed for specifically binding to the spike protein on MERS-NVs and it is prepared using the systematic evolution of ligands by exponential enrichment (SELEX) technique. For constructing a multi-functional MERS aptamer (MF-aptamer), the prepared aptamer was connected to the DNA 3-way junction (3WJ) structure. DNA 3WJ has the three arms that can connect the three individual functional groups including MERS aptamer (bioprobe), methylene blue (signal reporter) and thiol group (linker) Then, GO-MoS2 hybrid nanocomposite was prepared for the substrate of EC/SERS-based MERS-NV biosensor construction. Then, the assembled multifunctional (MF) DNA aptamer was immobilized on GO-MoS2. The proposed biosensor can detect MERS-NVs not only in a phosphate-buffered saline (PBS) solution (SERS LOD: 0.176 pg/ml, EIS LOD: 0.405 pg/ml) but also in diluted 10% saliva (SERS LOD: 0.525 pg/ml, EIS LOD: 0.645 pg/ml).
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