期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 348, 期 -, 页码 -出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2021.130677
关键词
Fluorescent probe; Head and neck squamous cell carcinoma (HNSCC); Targeting strategy; Sodium-dependent multivitamin transporter (SMVT); Peroxynitrite (ONOO-)
资金
- Department of Human Resources and Social Security of Shandong Province [202002050]
- China Postdoctoral Science Foundation [2019M652380]
- Project of Shandong Province Higher Educational Science and Technology Support Program for Young Innovation [2019KJD005]
- National Natural Science Foundation of China [81901022]
The study developed a novel fluorescent probe RB-PN for precise detection of HNSCC, which can be applied in tumor visualization and cytokine therapy. The probe with a dual-targeting strategy has rapid response, high specificity, and selectivity.
Head and neck squamous cell carcinoma (HNSCC) is the 6th most common cancer worldwide. For better diagnosis and precise resection of HNSCC, Fluorescent probe has been developed as an ideal tool to visualize tumor sites and to detect biological molecules. However, the targeting strategies of small molecular probes are limited. We designed probe RB-PN (Rhodamine biotin-peroxynitrite) with both sodium-dependent multivitamin transporter (SMVT)-targetable and peroxynitrite (ONOO-)-activatable tumor-targeting strategies. It can detect ONOO- with high specificity and selectivity, and rapid response. Besides, it exhibits prominent imaging abilities with enhanced accumulation by SMVT-transportation. The remarkable sensitivity, selectivity, turn-on signal change and rapid responsive time have been identified. We evidenced the dual-targeting strategy, and applied it in tumor visualization and ONOO- detection both in HNSCC cells and in vivo. We also identified the potential application of it in cytokine therapy using 3D micro-spheroid models. We expect RB-PN with a dual-targeting strategy could be conducive to the diagnosis of tumor pre-operatively or even intra-operatively.
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